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The present study was performed to investigate competitive interaction between arenobufagin and verapamil hydrochloride with serum albumin.
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<p><b>OBJECTIVE</b>To measure the contents of the water-soluble iron, five heavy metals and harmful elements in Magnetiturn and provide a basis for the quality control and safety evaluation of Magnetitum.</p><p><b>METHOD</b>Iron (Fe), lead (Pb), cadmium (Cd) and copper (Cu) were determined by atomic absorption spectrometry (AAS); arsenic (As) and mercury (Hg) were determined by atomic fluorescence spectrometry (AFS).</p><p><b>RESULT</b>The mean content of element iron is 764.30 mg x kg(-1). The contents of five water-soluble heavy metals and harmful elements in Magnetitum were within the safety range. The recovery of the standard addition was in the range of 93.7% - 110.6%, and the RSD was less than 5.0%.</p><p><b>CONCLUSION</b>Analyzing the water-soluble iron, heavy metals and harmful elements in Magnetitum is effective to the quality control and the safety evaluation of magnetitum.</p>
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Iron , Metabolism , Materia Medica , Chemistry , Metals, Heavy , Metabolism , Solubility , Spectrophotometry, AtomicABSTRACT
The technology of powder X-ray diffraction (XRD) was used for analysis Chloriti Lapis and the XRD Fourier fingerprints were established. The dates were analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples. XRD fingerprint with 10 common peaks of 14 batches of Chloriti Lapis were established. The average, median coefficients of crystal lattice spacing d (A), peak position 2 theta, relative intensity value I/I0 (%) were all more than 0.95. And similarity( angle cosine value) were all more than 0. 97. There were small number samples differed from others. And obvious differences between the pre-and post-processing samples. This paper shows the powder XRD Fourier fingerprint can be used for appraisal and study of the Chloriti Lapis.
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Aluminum Silicates , Chemistry , Cluster Analysis , Drugs, Chinese Herbal , Chemistry , Ferrous Compounds , Chemistry , Fourier Analysis , Geography , Medicine, Chinese Traditional , X-Ray Diffraction , MethodsABSTRACT
<p><b>OBJECTIVE</b>To evaluate the bio-activity of Qinlian Siwu decoction on in vitro uterus contraction model and exploit the relationship between chemical components and the bio-activity.</p><p><b>METHOD</b>The samples were prepared by macroporous adsorptive resins. The in vitro uterus contraction model was adopted to appraise the bio-activities of Qinlian Siwu decoction and its different separated fractions. HPLC-DAD- ESI -MS method was applied to analyze and identify the components in the fraction QL-3.</p><p><b>RESULT</b>It was found that five active fractions (QL-1, QL-3, QL-5, QL-7 and QL-11) were separated from Qinlian Siwu decoction, mainly contributed to the observed antagonismto the contraction of the mouse uterus. 28 compounds in the fraction QL-3 were identified as malic acid, gallic acid, catalpol, protocatechuic acid, aucubin, chuanxiongzine hydrochloridum, vanillic acid, caffeic acid, paeoniflorin, berberastine, albiflorin, tetrahydropalmatine, coptisine, jatrorrhizine, leonuride, worenine, ferulic acid, palmatine, berberine, scutellarin, baicalin-7-0-glucoside, baicalin, rehmannioside C, wogonoside, chrysin-7-glucuronide, ttetuin, baicalein, wogonin and oroxylin-A.</p><p><b>CONCLUSION</b>In vitro inhibiting the contraction of the isolated mouse uterine of Qinlian Siwu decoction was mainly attributed to the fraction QL-1 and QL-3. The active fractions (QL-5, QL-7 and QL-11) were obtained from QL-3 on the macroporous adsorptive resins by the gradient elution using ethanol.</p>
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Animals , Female , Mice , Drugs, Chinese Herbal , Chemistry , Pharmacology , Mass Spectrometry , Muscle, Smooth , Physiology , Statistics as Topic , Methods , Uterine Contraction , Uterus , PhysiologyABSTRACT
<p><b>OBJECTIVE</b>To evaluate the correlativity between volatile components of Wuao decoction and its major constituting herbs.</p><p><b>METHOD</b>The chemical compositions of essential oil, obtained by hydrodistillation from Wu-ao Decoction and its major constituting herbs (Herba Ephedrae, Semen Armeniacae Amarum, Radix Platycodi, Herba Schizonepetae), were analyzed by GC-MS.</p><p><b>RESULT</b>The volatile components of Wu-ao Decoction were mostly derived from Herba Ephedrae, Radix Platycodi and Herba Sehizonepetae.</p><p><b>CONCLUSION</b>The method of GC-MS can be used to investigate the volatile component changes in traditional Chinese medicine formulae.</p>
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Asteraceae , Chemistry , Drugs, Chinese Herbal , Chemistry , Gas Chromatography-Mass Spectrometry , Methods , Oils, Volatile , ChemistryABSTRACT
Objective To study on the quality of Eucommia ulmodies processing in planting by different methods.Methods The quantity percent of coarsed cortex,water and ethanol extract were compred,the contents of Pinoresinol diglucoside(PDG)were determined by HPLC,the residues of pesticides and the content of heavy metal element were carried out to investigate the quality.Results The quantity percent of coarsed cortex was 12.7%(n=3).Ethanol extract had no remarkable difference whether scrape the coarsed cortex or not.Water extract was higher 11.26%(P
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Objective The methods for determination of Chlorogenic Acid(CA) in Eucommia ulmodies Oliv were established and determinated the content of CA. Extract solvent, method, time and times were studied and HPLC method for determination was used. Methods The HPLC system consisted of the column of Lichrospher C18 (4.6 mm?250 mm, 5 ?m) and acetonitrtle-water-phosphoric acid (13∶86.5∶ 0.5) as the mobile phase. The temperature was 25℃. The detective wavelength was 327nm. The flow rate was 0.8 mL/min. External standard method was used. Results The powder of eucommia was extracted twice with 60%methanol-water solution at 100 ℃, the first time for 1.5 h,the second for 0.5 h was the optimum one. The method was linear within the range of 2.22?10-2～55.40?10-2 ?g, the average recovery was 99.55%, and the RSD was 1.98% (n =6). Conclusion The method is simple, sensitive and highly reproductive, and it is suitable for ananlysis of CA in Eucommia ulmodies Oliv.
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OBJECTIVE:To establish the quality standards for Weianning granules.METHODS:Radix Astragali,Hedyotic Diffusa,Radix Sophorae in Weianning granules were identified with TLC;Astragaloside Ⅳ in the granules was determined by HPLC-ELSD.RESULTS:The TLC spots were clear with high resolution.The linear range of Astragaloside Ⅳ was 2.567～12.840 ?g(r=0.999 6),with average recovery rate at 99.63%(RSD=4.69%,n=6).CONCLUSION:The est-ablished standards are suitable for the quality control of Weianning granules.
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Various properties of the raw drug Horsc Bozoar were studied by microscopic observation, X-ray phase analysis, thermal analysis, elementary analysis and solubility test .Resul.ts showed that Horse Bezoar mainly contains magnesium ammonium phosphate as well assome magnesium -phosphorus stones.